Journal: PloS one

Article Title: Activation of GSK-3β and caspase-3 occurs in Nigral dopamine neurons during the development of apoptosis activated by a striatal injection of 6-hydroxydopamine.

doi: 10.1371/journal.pone.0070951

Figure Lengend Snippet: Figure 2. Representative merged micrographs of mesencephalon immunostained against NeuN and TH. The numbers at the left margin of the figures are the days after 6-OHDA lesion. The primary antibodies were a mouse monoclonal anti-NeuN and a rabbit polyclonal anti-TH. The secondary antibodies were sheep anti-mouse IgG (H+L) FITC conjugated and goat anti-rabbit IgG (H+L) Texas conjugated. The rectangles on the panoramic view show the regions that were amplified 40x (details). Scale bar = 1 mm (central panels) and 50 mm (details). doi:10.1371/journal.pone.0070951.g002

Article Snippet: The secondary antibodies were fluorescein isothiocyanate (FITC) sheep anti- mouse H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), Texas red horse anti-mouse H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), FITC goat anti-rabbit H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), PLOS ONE | www.plosone.org 3 August 2013 | Volume 8 | Issue 8 | e70951 Texas red goat anti-rabbit H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), and Alexa 488 chicken anti-mouse (1:200; Invitrogen Molecular Probes; Eugene, Oregon, USA).

Techniques: Amplification

Journal: PloS one

Article Title: Activation of GSK-3β and caspase-3 occurs in Nigral dopamine neurons during the development of apoptosis activated by a striatal injection of 6-hydroxydopamine.

doi: 10.1371/journal.pone.0070951

Figure Lengend Snippet: Figure 3. Neuronal cytoskeleton loss occurs along with the progressive loss of TH(+) cells in the SNc after the 6-OHDA injection into the neostriatum. In panel A, representative micro- graphs of mesencephalon with double immunostaining to b-III tubulin and TH. The numbers at the left margin of the figures show the days after the 6-OHDA lesion. The primary antibodies were a mouse anti-TH monoclonal and a rabbit anti-b-III tubulin polyclonal. The secondary antibodies were sheep anti-mouse IgG (H+L) FITC conjugated and goat anti-rabbit IgG (H+L) Texas conjugated. In panel B, representative micrographs of SNc showing details of the loss of b-tubulin III immunoreactivity at days 21 and 30 after the 6-OHDA injection as compared with the intact control side. Scale bars = 20 mm. The scale bars in the panel A are common for all the micrographs. doi:10.1371/journal.pone.0070951.g003

Article Snippet: The secondary antibodies were fluorescein isothiocyanate (FITC) sheep anti- mouse H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), Texas red horse anti-mouse H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), FITC goat anti-rabbit H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), PLOS ONE | www.plosone.org 3 August 2013 | Volume 8 | Issue 8 | e70951 Texas red goat anti-rabbit H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), and Alexa 488 chicken anti-mouse (1:200; Invitrogen Molecular Probes; Eugene, Oregon, USA).

Techniques: Injection, Double Immunostaining, Control

Journal: PloS one

Article Title: Activation of GSK-3β and caspase-3 occurs in Nigral dopamine neurons during the development of apoptosis activated by a striatal injection of 6-hydroxydopamine.

doi: 10.1371/journal.pone.0070951

Figure Lengend Snippet: Figure 5. The injection of 6-OHDA into the neostriatum leads to the activation of caspase-3 in the SNc. Representative confocal micrographs of mesencephalon immunostained against TH and cleaved caspase-3. The numbers at the left margin of the figures are the days after the 6-OHDA lesion. The primary antibodies were a mouse monoclonal anti-TH and a rabbit polyclonal anti-cleaved-caspase-3. The secondary antibodies were FITC goat anti-mouse IgG (H+L) conjugated and Texas red goat anti-rabbit H+L IgG. The scale bars = 50 mm in the first row are common for all the micrographs. doi:10.1371/journal.pone.0070951.g005

Article Snippet: The secondary antibodies were fluorescein isothiocyanate (FITC) sheep anti- mouse H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), Texas red horse anti-mouse H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), FITC goat anti-rabbit H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), PLOS ONE | www.plosone.org 3 August 2013 | Volume 8 | Issue 8 | e70951 Texas red goat anti-rabbit H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), and Alexa 488 chicken anti-mouse (1:200; Invitrogen Molecular Probes; Eugene, Oregon, USA).

Techniques: Injection, Activation Assay

Journal: PloS one

Article Title: Activation of GSK-3β and caspase-3 occurs in Nigral dopamine neurons during the development of apoptosis activated by a striatal injection of 6-hydroxydopamine.

doi: 10.1371/journal.pone.0070951

Figure Lengend Snippet: Figure 7. Increase of the GSK-3b pY216 immunoreactivity in the substantia nigra after a striatal 6-OHDA injection. In panel A, representative confocal micrographs of the SNc double immunostained against TH and GSK-3b pY216 at different days (shown at the left margin) after the 6-OHDA striatal injection. In B, the micrographs in the Details column correspond to amplification of the area within the corresponding rectangles on the panoramic view. The primary antibodies were a rabbit polyclonal anti-TH and a mouse monoclonal anti-GSK-3b pY216. The secondary antibodies were fluorescein isothiocyanate (FITC) goat anti-rabbit H+L IgG and Texas red horse anti-mouse H+L IgG. The scale bars = 50 mm of the first row are common for all the micrographs. doi:10.1371/journal.pone.0070951.g007

Article Snippet: The secondary antibodies were fluorescein isothiocyanate (FITC) sheep anti- mouse H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), Texas red horse anti-mouse H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), FITC goat anti-rabbit H+L IgG (1:60; Jackson Immunoresearch; West Grove, PA, USA), PLOS ONE | www.plosone.org 3 August 2013 | Volume 8 | Issue 8 | e70951 Texas red goat anti-rabbit H+L IgG (1:300; Vector laboratories; Burlingame, CA, USA), and Alexa 488 chicken anti-mouse (1:200; Invitrogen Molecular Probes; Eugene, Oregon, USA).

Techniques: Injection, Amplification

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Curcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in Carcinogenesis

doi: 10.1155/2012/593952

Figure Lengend Snippet: Effect of curcumin on UVB-induced p53 and p21/Cip1 expressions. CUR enhances UVB-induced p53 and p21/Cip1 expressions, left and right panels, respectively. (a) and (e) controls; (b) and (f) UV; (c) (g) CUR-T + UV. Arrows show p53-positive cells ((b) and (c)) or p21/Cip1-positive cells ((f) and (g)) with brown staining. (d) and (h) are the quantitative results of p53- and p21/Cip1-positive cells cell populations in five different experimental conditions, respectively. * P < 0.004 (p53), * P < 0.003 (p21/Cip1).

Article Snippet: For detection of p53, p21/Cip1,and PCNA, mouse monoclonal anti-p53 (LifeSpan BioSciences, Seattle, WA, USA), anti-p21/Cip1 (Acris Antibodies GmbH, Herford, Germany), or anti-PCNA (Biocare Medical, Concord, CA, USA) antibodies were used.

Techniques: Staining